T3824

Sigma

 

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

Synonym:Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
CAS Number:9002-10-2
Enzyme Commission (EC) Number:1.14.18.1   ( BRENDA | IUBMB )
EC Number:232-653-4
MDL number:MFCD00082118

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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol ActionsConverts tyrosine to L-DOPA or tyramine to dopamine
Unit DefinitionOne unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.
General descriptionIsoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.

Properties

formlyophilized powder
storage temp.−20°C

Safety

Safety Statements22-24/25
WGK Germany2

References

MerckMerck 13,9906